The Phaff Yeast Culture Collection accepts strains for deposit that will be made available to the research community. These include for example type strains of new species, yeasts used in genome sequencing projects, or other strains that the depositor would like to make available to the broader yeast research community.

Here’s how the strain deposit process works: The Deposit Agreement and Strain Deposit Worksheet (see below) are first completed by the Depositor, emailed to the Phaff collection and approved by the curator. The Phaff collection will confirm how many and which strains will be accepted for deposit. After this is complete, then yeast strains can be shipped to UC Davis. The Phaff collection accepts strains under these conditions:

• The yeast strain is pure, viable, and identified by ribosomal or other DNA sequencing.
• It is not a known human, plant or animal pathogen.
• The depositor and/or their institutional representative completes and signs the Deposit Agreement. Note that if the depositor wishes to make any edits, they must be approved by both the University of California and the depositor’s institution.
• Data associated with the yeast strain is provided either within the Deposit Agreement or by filling in the Strain Deposit Worksheet Note that sample text is provided. Please delete this and replace with text related to your strains, one strain per column. Fields in green are required, and other fields are various levels of optional. For more information about data formats, hover over the red triangle in the corner of the cell in the spreadsheet. We greatly appreciate depositors carefully filling out this spreadsheet as it streamlines the process of importing data into our website, helps prevent errors in data entry, and ensures that the data are in a format that will be useful to future users of the Phaff collection!

After the forms have been submitted and approved by Phaff collection personnel, then yeasts can be shipped to UC Davis using the shipping addresses at the bottom of the Phaff collection home page. The depositor will prepare samples in a format that is compatible with shipping methods. The yeasts are then revived, viability and purity are confirmed. If species ID has not been performed before deposit, then ribosomal or other DNA sequencing will be performed at the expense of the Depositor. The yeast will be cryopreserved in triplicate: working and backup stocks in the -80C freezers at UC Davis, and an archived stock at a remote cryopreservation facility.

The Phaff collection can delay release of yeast strains into the public catalog at the request of the depositor. Please contact that Phaff collection if you have questions.